anti abcg1 (Novus Biologicals)
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Novus Biologicals
anti abcg1
Anti Abcg1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abcg1/ABCG1+Antibody/pm41898701-387-61-62
Average 94 stars, based on 27 article reviews
Anti Abcg1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abcg1/ABCG1+Antibody/pm41898701-387-61-62
Average 94 stars, based on 27 article reviews
anti abcg1 - by Bioz Stars,
2026-09
94/100 stars
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Incubation:Article Title: Imeglimin Inhibits Macrophage Foam Cell Formation and Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-Deficient Mice. Article Snippet: Equal amounts of protein (20 μg) were loaded onto NuPAGETM 4–12% Bis-Tris gradient gels (NOVEX by Life Technologies, Winston-Salem, NC, USA) and electrophoresed before being transferred onto nitrocellulose membranes. .. Membranes were blocked using 5% bovine serum albumin at room temperature for 1 h, followed by overnight incubation at 4 ◦C with primary antibodies targeting CD36 (PA1-16813, 1:1000, Thermo Fisher Scientific), ABCA1 (NB400-105, 1:1000, Novus Biologicals, Littleton, CO, USA), Article Title: Luteolin Nanomedicine with Stimulus-Driven Traceless Release for Targeting Treatment of Atherosclerosis by Enhancing Lipid Efflux Article Snippet: .. The membranes were blocked with 5% bovine serum albumin (BSA) in PBS for 1 h at room temperature and subsequently incubated overnight at 4 °C with specific primary antibodies, such as ABCA1 (Novus, USA) and Article Title: Ex Vivo Characterization of Peritoneal Macrophages from Novel ABCA1-LSL and ABCG1-LSL Mice for Macrophage-Specific ABC-Transporter Overexpression Article Snippet: We calculated the protein concentrations [ ] in PM lysates with a BCA assay (BioVision, Milpitas, CA, USA), and we loaded an equal mass of protein (1 μg) from lysates onto SDS-PAGE gels that were used for protein separation. .. We then transferred separated proteins onto PVDF membranes (Merck Millipore, Burlington, MA, USA), incubated the PVDF membranes in blocking buffer [ ], and then probed for either ABCA1 (1:500 dilution, sc-58219; Santa Cruz Biotechnology, Dallas, TX, USA) or Article Title: Imeglimin Inhibits Macrophage Foam Cell Formation and Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-Deficient Mice Article Snippet: Equal amounts of protein (20 μg) were loaded onto NuPAGETM 4–12% Bis-Tris gradient gels (NOVEX by Life Technologies, Winston-Salem, NC, USA) and electrophoresed before being transferred onto nitrocellulose membranes. .. Membranes were blocked using 5% bovine serum albumin at room temperature for 1 h, followed by overnight incubation at 4 °C with primary antibodies targeting CD36 (PA1-16813, 1:1000, Thermo Fisher Scientific), ABCA1 (NB400-105, 1:1000, Novus Biologicals, Littleton, CO, USA), Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis Article Snippet: Protein was extracted in RIPA buffer (Cell Signaling) with protease and phosphatase inhibitors (Roche) and subsequently normalized with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). .. Samples (30 μg per well) were electrophoresed on 4–20% TGX-gradient gels (Bio-Rad Laboratories) and transferred to nitrocellulose membranes at 125 V for 2 h. Membranes were incubated overnight with the specified antibodies directed against COX-2 (Cayman 160106) ABCA1 (Novus Biologicals, NB400-105), Western Blot:Article Title: Targeting the ARF6-dependent recycling pathway to alter lipid rafts and reduce inflammation. Article Snippet: .. Semi-quantitative analysis of western blots was performed by densitometry and presented as a proportion of control after normalization to loading controls .Antibodies used in western blot analysis: ABCA1 (Abcam, #ab18180, 1:3000), ATPase (Abcam, #ab76671, 1:5000), GAPDH (Merck, #CB1001, 1:5000), Arf6 (SigmaAldrich, #A5230, 1:2000), Cdc42(BD transduction Laboratories, #610929, 1:500), Erk1/2 (Millipore, #66192, 1:10000), pErk1/2 (Cell Signaling Tech.#4370, 1:2000), TMS1(Cell Signaling Jo urn al Pr e-p roo f 11 Tech.#67824 1:1000), NRLP3 (Cell Signaling Tech.#15101, 1:1000), Caspase-1 ((Cell Signaling Tech.#24232, 1:1000), Control:Article Title: Targeting the ARF6-dependent recycling pathway to alter lipid rafts and reduce inflammation. Article Snippet: .. Semi-quantitative analysis of western blots was performed by densitometry and presented as a proportion of control after normalization to loading controls .Antibodies used in western blot analysis: ABCA1 (Abcam, #ab18180, 1:3000), ATPase (Abcam, #ab76671, 1:5000), GAPDH (Merck, #CB1001, 1:5000), Arf6 (SigmaAldrich, #A5230, 1:2000), Cdc42(BD transduction Laboratories, #610929, 1:500), Erk1/2 (Millipore, #66192, 1:10000), pErk1/2 (Cell Signaling Tech.#4370, 1:2000), TMS1(Cell Signaling Jo urn al Pr e-p roo f 11 Tech.#67824 1:1000), NRLP3 (Cell Signaling Tech.#15101, 1:1000), Caspase-1 ((Cell Signaling Tech.#24232, 1:1000), Transduction:Article Title: Targeting the ARF6-dependent recycling pathway to alter lipid rafts and reduce inflammation. Article Snippet: .. Semi-quantitative analysis of western blots was performed by densitometry and presented as a proportion of control after normalization to loading controls .Antibodies used in western blot analysis: ABCA1 (Abcam, #ab18180, 1:3000), ATPase (Abcam, #ab76671, 1:5000), GAPDH (Merck, #CB1001, 1:5000), Arf6 (SigmaAldrich, #A5230, 1:2000), Cdc42(BD transduction Laboratories, #610929, 1:500), Erk1/2 (Millipore, #66192, 1:10000), pErk1/2 (Cell Signaling Tech.#4370, 1:2000), TMS1(Cell Signaling Jo urn al Pr e-p roo f 11 Tech.#67824 1:1000), NRLP3 (Cell Signaling Tech.#15101, 1:1000), Caspase-1 ((Cell Signaling Tech.#24232, 1:1000), Blocking Assay:Article Title: Ex Vivo Characterization of Peritoneal Macrophages from Novel ABCA1-LSL and ABCG1-LSL Mice for Macrophage-Specific ABC-Transporter Overexpression Article Snippet: We calculated the protein concentrations [ ] in PM lysates with a BCA assay (BioVision, Milpitas, CA, USA), and we loaded an equal mass of protein (1 μg) from lysates onto SDS-PAGE gels that were used for protein separation. .. We then transferred separated proteins onto PVDF membranes (Merck Millipore, Burlington, MA, USA), incubated the PVDF membranes in blocking buffer [ ], and then probed for either ABCA1 (1:500 dilution, sc-58219; Santa Cruz Biotechnology, Dallas, TX, USA) or |
